Site-directed mutagenesis and CBM engineering of Cel5A (Thermotoga maritima)
نویسندگان
چکیده
منابع مشابه
Correction: Site-Directed Mutagenesis of a Hyperthermophilic Endoglucanase Cel12B from Thermotoga maritima Based on Rational Design
Fig 4 is incorrect. Fig 4 incorrectly appears a duplicate of Fig 5. The authors have provided a corrected version of Fig 4 here. Fig 4. Structures of site-directed amino acid residues with other vicinal residues by H-bond. Copyright: © 2015 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distrib...
متن کاملSite-Directed Mutagenesis of a Hyperthermophilic Endoglucanase Cel12B from Thermotoga maritima Based on Rational Design
To meet the demand for the application of high activity and thermostable cellulases in the production of new-generation bioethanol from nongrain-cellulose sources, a hyperthermostable β-1,4-endoglucase Cel12B from Thermotoga maritima was selected for further modification by gene site-directed mutagenesis method in the present study, based on homology modeling and rational design. As a result, t...
متن کاملImproved Activity of a Thermophilic Cellulase, Cel5A, from Thermotoga maritima on Ionic Liquid Pretreated Switchgrass
Ionic liquid pretreatment of biomass has been shown to greatly reduce the recalcitrance of lignocellulosic biomass, resulting in improved sugar yields after enzymatic saccharification. However, even under these improved saccharification conditions the cost of enzymes still represents a significant proportion of the total cost of producing sugars and ultimately fuels from lignocellulosic biomass...
متن کاملDirected Evolution of a Hyperthermophilic Endoglucanase Cel12B from Thermotoga maritima
The cel12B gene was cloned, optimized through directed evolution using error-prone polymerase chain reaction, and then expressed in the Escherichia coli BL21 (DE3) host strain. Five mutants promoting the enzyme activities were selected. The specific activity of the best-evolved Cel16 (L20R, D37V, I108T) was improved approximately 3-fold compared to the parental enzyme. The residual enzyme activ...
متن کاملModulation of the regioselectivity of a Thermotoga neapolitana beta-glucosidase by site-directed mutagenesis.
Thermotoga neapolitana beta-glucosidase (BglA) was subjected to site-directed mutagenesis in an effort to increase its ability to synthesize arbutin derivatives by transglycosylation. The transglycosylation reaction of the wild-type enzyme displays major beta(1,6) and minor beta(1,3) or beta(1,4) regioselectivity. The three mutants, N291T, F412S, and N291T/F412S, increased the ratio of transgly...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2008
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2008.01324.x